Anticorps Monoclonal anti-NANOG

NANOG Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

10

CloneNo.

3A2E1

N° de cat : 67255-1-Ig

Synonymes

hNanog, Homeobox protein NANOG, NANOG, Nanog homeobox

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Galerie de données de validation

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  WB analysis using 67255-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67255-1-Ig (NANOG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules HuH-7, cellules JAR, cellules MCF-7, cellules MDA-MB-231, cellules NCCIT, cellules T-47D, tissu placentaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below

Informations sur le produit

67255-1-Ig cible NANOG dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NANOG Protéine recombinante Ag21364
• Nom complet: Nanog homeobox
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC160187
• Symbole du gène: NANOG
• Identification du gène (NCBI): 79923
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. Affinity purified rabbit anti-Nanog can be used to demonstrate pluripotency of ES and IPS cells. There are two kinds of variants could recognized by NANOG, one is normal form (~39kd), the other is post-translation modified form (~48kd) (21136380 ). Nanog exists two isoforms with molecular weight 34.4 kDa and 31.9 kDa. (PMID: 21969378)

Publications

Species	Application	Title
human	WB	iScience; Arl4c promotes the growth and drug resistance of pancreatic cancer by regulating tumor-stromal interactions.; Authors - Xin Chen
human	WB	Biomed Pharmacother; Limonin enhances the radiosensitivity of nasopharyngeal carcinoma cells via attenuating Stat3-induced cell stemness.; Authors - Ling Gao
human	WB	Med Sci Monit; PMID: 31760402; Authors - Xueni Liu
human	WB	Evid Based Complement Alternat Med; Antitumor Effect of Si-Jun-Zi Decoction on SGC7901 Gastric Cancer Cells by CMTM2 Activation; Authors - Xiangnan Li
human	WB	Clin Transl Med; The driver role of JAK-STAT signalling in cancer stemness capabilities leading to new therapeutic strategies for therapy- and castration-resistant prostate cancer.; Authors - U‐Ging Lo
human	WB	Apoptosis; NRP1 contributes to stemness and potentiates radioresistance via WTAP-mediated m6A methylation of Bcl-2 mRNA in breast cancer; Authors - Yang Wang