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Anticorps Monoclonal anti-Nanog

Nanog Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3A2E1

N° de cat : 67255-1-PBS

Synonymes

hNanog, 3A2E1

Galerie de données de validation

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  • Western Blot (WB) analysis of various lysates using Nanog Monoclonal antibody (67255-1-Ig)

    WB analysis using 67255-1-Ig (same clone as 67255-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67255-1-Ig (Nanog antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67255-1-PBS in a different storage buffer formulation.

Informations sur le produit

67255-1-PBS cible Nanog dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Mouse / IgG2a
Clonalité Monoclonal
Type Anticorps
Immunogène Nanog Protéine recombinante Ag21364
Nom complet Nanog homeobox
Masse moléculaire calculée 35 kDa
Poids moléculaire observé35-40 kDa
Numéro d’acquisition GenBankBC160187
Symbole du gène Nanog
Identification du gène (NCBI) 79923
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. Affinity purified rabbit anti-Nanog can be used to demonstrate pluripotency of ES and IPS cells. There are two kinds of variants could recognized by NANOG, one is normal form (~39kd), the other is post-translation modified form (~48kd) (21136380 ). Nanog exists two isoforms with molecular weight 34.4 kDa and 31.9 kDa. (PMID: 21969378)

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