Anticorps Recombinant de lapin anti-Nanog

Nanog Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

250067F10

N° de cat : 80210-13-PBS

Synonymes

hNanog

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Galerie de données de validation

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  WB analysis using 80210-13-RR (same clone as 80210-13-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80210-13-RR (NANOG antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

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  WB analysis using 80210-13-RR (same clone as 80210-13-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80210-13-RR (NANOG antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02157-2

  Sandwich ELISA standard curve of MP02157-2, Human Nanog Recombinant Matched Antibody Pair - PBS only. 80210-13-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag5645. 80210-12-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  Sample test of MP02157-2

  The mean Nanog concentration was determined to be 9.25 ng/mL in NCCIT cell extract based on a 1.50 mg/mL extract load.

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  IHC staining of mouse brain using 80210-13-RR (same clone as 80210-13-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80210-13-RR (Nanog antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

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  IHC staining of mouse embryo using 80210-13-RR (same clone as 80210-13-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 80210-13-RR (Nanog antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

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  IHC staining of mouse embryo using 80210-13-RR (same clone as 80210-13-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 80210-13-RR (Nanog antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 80210-13-RR (same clone as 80210-13-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80210-13-RR (Nanog antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

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  IF Staining of NCCIT using 80210-13-RR (same clone as 80210-13-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using Nanog antibody (80210-13-RR, Clone: 250067F10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80210-13-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02157-2

  Cytometric bead array standard curve of MP02157-2, Nanog Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80210-13-PBS. Detection antibody: 80210-12-PBS. Standard: Ag5645. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 80210-13-RR

  Biolayer interferometry (BLl) kinetic assays of 80210-13-RR against Human Nanog were performed. The affinity constant is 11.0 nM.

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Informations sur le produit

80210-13-PBS cible Nanog dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Nanog Protéine recombinante Ag5645
• Nom complet: Nanog homeobox
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC160187
• Symbole du gène: Nanog
• Identification du gène (NCBI): 79923
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. There are two kinds of variants that can be recognized by NANOG, one is a normal form (~39 kDa), the other is a post-translation modified form (~48 kDa) (21136380 ). Nanog has two isoforms with molecular weights of 34.4 kDa and 31.9 kDa. (PMID: 21969378)

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