Anticorps Recombinant de lapin anti-NAP1;NCKAP1

NAP1;NCKAP1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242784C5

N° de cat : 85142-2-RR

Synonymes

NCKAP1, NAP1, NAP 1, Membrane-associated protein HEM-2, KIAA0587

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Galerie de données de validation

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  WB analysis using 85142-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85142-2-RR (NAP1;NCKAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85142-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85142-2-RR against Human NAP1;NCKAP1 were performed. The affinity constant is 0.116 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293T, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85142-2-RR cible NAP1;NCKAP1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NAP1;NCKAP1 Protéine recombinante Ag2788
• Nom complet: NCK-associated protein 1
• Masse moléculaire calculée: 129 kDa
• Poids moléculaire observé: 115-125 kDa
• Numéro d’acquisition GenBank: BC015025
• Symbole du gène: NCKAP1
• Identification du gène (NCBI): 10787
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NCK-associated protein 1 (NCKAP1), also known as NAP1 or HEM2, is encoded by an apoptosis-related gene human NAP1, the expression of which is strongly down-regulated in sporadic Alzheimer's disease (AD). Human NAP1 is predominantly expressed in neuronal cells. Antisense oligo DNA of human NAP1 transcripts was found to induce apoptosis of neuronal cells. Loss of NAP1 function disrupts neuronal differentiation. Likewise, NAP1 plays an essential role in facilitating neuronal cytoskeletal changes underlying the postmigratory differentiation of cortical neurons, a critical step in functional wiring of the cortex. Human NAP1 is also proved to be an orthologue of rat Nap1 which binds to the adaptor molecule NCK in signal transduction.

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