Anticorps Polyclonal de lapin anti-NAPB

NAPB Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14106-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 14106-1-AP (same clone as 14106-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14106-1-AP (NAPB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 14106-1-AP (same clone as 14106-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14106-1-AP (NAPB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 14106-1-AP (same clone as 14106-1-PBS)

  IP result of anti-NAPB (IP:14106-1-AP, 4ug; Detection:14106-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14106-1-AP (same clone as 14106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14106-1-AP (NAPB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 14106-1-AP (same clone as 14106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14106-1-AP (NAPB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 14106-1-AP (same clone as 14106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14106-1-AP (NAPB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14106-1-AP (same clone as 14106-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using NAPB antibody 14106-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14106-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14106-1-PBS cible NAPB dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NAPB Protéine recombinante Ag5239
• Nom complet: N-ethylmaleimide-sensitive factor attachment protein, beta
• Masse moléculaire calculée: 298 aa, 34 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC060840
• Symbole du gène: NAPB
• Identification du gène (NCBI): 63908
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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