Anticorps Monoclonal anti-NAPRT1

• Phare
• Validé par KD/KO

NAPRT1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

5D8H10

N° de cat : 66159-1-PBS

Synonymes

NAPRT, FHIP, FHA-HIT-interacting protein, FHA HIT interacting protein, EC:6.3.4.21

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Galerie de données de validation

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  WB analysis using 66159-1-Ig (same clone as 66159-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  WB analysis using 66159-1-Ig (same clone as 66159-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 cells using 66159-1-Ig (same clone as 66159-1-PBS)

  WB result of NAPRT1 antibody (66159-1-Ig, 1:6000) with si-Control and si-NAPRT1 transfected HepG2 cells. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66159-1-Ig (same clone as 66159-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66159-1-Ig (same clone as 66159-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 66159-1-Ig (same clone as 66159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 66159-1-Ig (same clone as 66159-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66159-1-Ig (NAPRT1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66159-1-Ig (same clone as 66159-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66159-1-Ig(NAPRT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66159-1-Ig (same clone as 66159-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NAPRT1 antibody (66159-1-Ig, Clone: 5D8H10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66159-1-Ig (same clone as 66159-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NAPRT1 antibody (66159-1-Ig, Clone: 5D8H10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66159-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66159-1-PBS cible NAPRT1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NAPRT1 Protéine recombinante Ag4265
• Nom complet: nicotinate phosphoribosyltransferase domain containing 1
• Masse moléculaire calculée: 514 aa, 55 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC032466
• Symbole du gène: NAPRT1
• Identification du gène (NCBI): 93100
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nicotinic acid (NA) is a coenzyme in cellular redox reactions, and is an essential component of metabolic pathways in all living cells. NAPRT1 (Nicotinate phosphoribosyltransferase) is essential for increasing cellular NAD levels and, thus, to prevent oxidative stress of cells. NAPRT1 converts Nicotinic acid (NA; niacin) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD).

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