Anticorps Polyclonal de lapin anti-NARG1

NARG1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 23845-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 23845-1-AP (same clone as 23845-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23845-1-AP (NARG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23845-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 23845-1-AP (same clone as 23845-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23845-1-AP (NARG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23845-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 23845-1-AP (same clone as 23845-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23845-1-AP (NARG1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23845-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 23845-1-AP (same clone as 23845-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23845-1-AP (NARG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23845-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 23845-1-AP (same clone as 23845-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 23845-1-AP (NARG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23845-1-PBS in a different storage buffer formulation.

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Informations sur le produit

23845-1-PBS cible NARG1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NARG1 Protéine recombinante Ag20842
• Nom complet: NMDA receptor regulated 1
• Masse moléculaire calculée: 866 aa, 101 kDa
• Poids moléculaire observé: 95-100 kDa
• Numéro d’acquisition GenBank: BC093928
• Symbole du gène: NARG1
• Identification du gène (NCBI): 80155
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NAA15 (N-alpha-acetyltransferase 15) is a subunit of both the NatA and NatE complexes, and its role is to position the catalytic subunits in close vicinity to the nascent polypeptides (PMID: 33557580). Human NAA15 encodes an 866 amino acid (~105 kDa) protein, NAA15, containing tetratricopeptide repeat domains and a putative bipartite nuclear localization signal (PMID: 29656860). NAA15 was implicated in human disease and loss-of-function NAA15 variants in large cohorts of patients with autism spectrum disorder and/or ID (PMID: 33103328).

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