Anticorps Monoclonal anti-NARS

NARS Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4G5G12

N° de cat : 67711-1-PBS

Synonymes

ASNRS, Asparagine tRNA ligase, asparaginyl tRNA synthetase, NARS, NARS1

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Galerie de données de validation

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  WB analysis using 67711-1-Ig (same clone as 67711-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67711-1-Ig (NARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67711-1-PBS in a different storage buffer formulation.

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  IHC staining of human urothelial carcinoma using 67711-1-Ig (same clone as 67711-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67711-1-Ig (NARS antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67711-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67711-1-Ig (same clone as 67711-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NARS antibody (67711-1-Ig, Clone: 4G5G12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67711-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67711-1-PBS cible NARS dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NARS Protéine recombinante Ag6656
• Nom complet: asparaginyl-tRNA synthetase
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: BC001687
• Symbole du gène: NARS
• Identification du gène (NCBI): 4677
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Asparagine-tRNA ligase (NARS) belongs to the class-II aminoacyl-tRNA synthetase family. Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn) (PubMed: 9421509). In addition to its essential role in protein synthesis, acts as a signaling molecule that induced migration of CCR3-expressing cells. The NARS levels were higher in tumor tissues compared with adjacent normal tissues, and positively associated with LN metastasis. NARS may serve as a potential biomarker for lung adenocarcinoma (ADC) diagnosis/prognosis (PMID: 27161446).

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