Anticorps Polyclonal de lapin anti-NARS2

• Phare
• Validé par KD/KO

NARS2 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF,ELISA

Conjugaison

Non conjugué

N° de cat : 15714-1-AP

Synonymes

AsnRS, Asparagine tRNA ligase, NARS2, SLM5

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Galerie de données de validation

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  WB analysis using 15714-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15714-1-AP (NARS2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15714-1-AP

  WB result of NARS2 antibody (15714-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NARS2 transfected HepG2 cells.

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  WB analysis of human liver using 15714-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15714-1-AP (NARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15714-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15714-1-AP (NARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15714-1-AP (NARS2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15714-1-AP (NARS2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 15714-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15714-1-AP (NARS2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 15714-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15714-1-AP (NARS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 15714-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 15714-1-AP (NARS2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules K-562, tissu cérébral humain, tissu hépatique humain, tissu rénal de souris
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque humain, tissu placentaire humain, tissu pulmonaire humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

15714-1-AP cible NARS2 dans les applications de WB, IHC, IF,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NARS2 Protéine recombinante Ag8345
• Nom complet: asparaginyl-tRNA synthetase 2, mitochondrial (putative)
• Masse moléculaire calculée: 477 aa, 54 kDa
• Poids moléculaire observé: 50-54 kDa
• Numéro d’acquisition GenBank: BC007800
• Symbole du gène: NARS2
• Identification du gène (NCBI): 79731
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.