Anticorps Polyclonal de lapin anti-NASP

• Phare
• Validé par KD/KO

NASP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 11323-1-AP

Synonymes

PRO1999, FLB7527

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Galerie de données de validation

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  WB analysis of mouse testis using 11323-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11323-1-AP (NASP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 11323-1-AP

  IP result of anti-NASP (IP:11323-1-AP, 5ug; Detection:11323-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IHC staining of human testis using 11323-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11323-1-AP (NASP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 11323-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11323-1-AP (NASP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lymphoma using 11323-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 11323-1-AP (NASP antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 11323-1-AP

  Immunofluorescent analysis of MCF-7 cells, using NASP antibody 11323-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

11323-1-AP cible NASP dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NASP Protéine recombinante Ag1824
• Nom complet: nuclear autoantigenic sperm protein (histone-binding)
• Masse moléculaire calculée: 788 aa, 85 kDa
• Poids moléculaire observé: 150 kDa, 70 kDa
• Numéro d’acquisition GenBank: BC010105
• Symbole du gène: NASP
• Identification du gène (NCBI): 4678
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NASP (nuclear autoantigenic sperm protein) is associated with DNA replication, cell proliferation and cell cycle progression through functioning as a Histone H1 binding protein that mediates histone transport to the nucleus. NASP had two isoforms, tNASP (testis type) and sNASP (somatic type). The tNASP was mainly expressed in testis, a variety of malignant tumors, stem cells and embryonic tissues, while sNASP existed in all somatic mitosis cells. This antibody can recognize both isoforms.

Publications

Species	Application	Title
human,mouse	WB	Genes Dev; The death-associated protein DAXX is a novel histone chaperone involved in the replication-independent deposition of H3.3.; Authors - Drané Pascal P
human	WB	Nat Struct Mol Biol; The program for processing newly synthesized histones H3.1 and H4.; Authors - Campos Eric I EI ; KD Validated
human	WB	J Cell Sci; Role of Template Activating Factor-I as a chaperone in linker histone dynamics.; Authors - Kato Kohsuke K
human,mouse,rat	WB,IHC	J Biol Chem; MicroRNA-29a inhibited epididymal epithelial cell proliferation by targeting nuclear autoantigenic sperm protein (NASP).; Authors - Ma Wubin W
rat	WB	J Proteome Res; Proteomic and Phosphoproteomic Analysis of Right Ventricular Hypertrophy in the Pulmonary Hypertension Rat Model; Authors - Ang Luo
human	WB	Int J Nanomedicine; Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity.; Authors - Qing Yuan

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