Anticorps Recombinant de lapin anti-NAT10

• Phare
• Validé par KD/KO

NAT10 Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5B9

N° de cat : 82585-1-PBS

Synonymes

N acetyltransferase 10, KIAA1709, EC:2.3.1.-, ALP, 5B9

---

Galerie de données de validation

• 

  WB analysis using 82585-1-RR (same clone as 82585-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82585-1-RR (NAT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 82585-1-RR (same clone as 82585-1-PBS)

  WB result of NAT10 antibody (82585-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAT10 transfected HeLa cells. This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse testis using 82585-1-RR (same clone as 82585-1-PBS)

  IP result of anti-NAT10 (IP:82585-1-RR, 4ug; Detection:82585-1-RR 1:5000) with mouse testis tissue lysate 1120 ug. This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82585-1-RR (same clone as 82585-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (82585-1-RR, Clone: 5B9 ) at dilution of 1:1900 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82585-1-RR (same clone as 82585-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NAT10 antibody (82585-1-RR, Clone: 5B9 ) at dilution of 1:1900 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 82585-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82585-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82585-1-RR against Human NAT10 were performed. The affinity constant is below 1 pM.

---

Informations sur le produit

82585-1-PBS cible NAT10 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NAT10 Protéine recombinante Ag4184
• Nom complet: N-acetyltransferase 10 (GCN5-related)
• Masse moléculaire calculée: 1025 aa, 116 kDa
• Poids moléculaire observé: 116 kDa
• Numéro d’acquisition GenBank: BC035558
• Symbole du gène: NAT10
• Identification du gène (NCBI): 55226
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NAT10 (N-acetyltransferase 10) is a nucleolar protein that is involved in regulation of telomerase activity, DNA damage response, and cytokinesis. It also plays a role in maintaining nuclear shape. Inhibition of NAT10 has been reported to rescue the misshapen nuclei in laminopathic cells via microtubule reorganization. The specificity of this antibody has been tested by siRNA (PMID: 24786082). NAT10 regulates mitotic cell fate by acetylating Eg5. NAT10 depletion results in multinuclear giant cells, which is the hallmark of mitotic catastrophe (PMID: 35210604). NAT10 plays a crucial role in carcinogenesis through influencing EMT, hypoxia, ribosomal biogenesis and overall, promoting translational efficiency. Recently, we reported that treating cancer cells with Remodelin, a small molecule inhibitor of NAT10, causes alteration in global lipid metabolism (PMID: 36149760).

{{ptg:RelatedPrimaryAntibodies}}