Anticorps Polyclonal de lapin anti-NAT12

NAT12 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25149-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 25149-1-AP (same clone as 25149-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25149-1-AP (NAT12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25149-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 25149-1-AP (same clone as 25149-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NAT12 antibody (25149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25149-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25149-1-PBS cible NAT12 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NAT12 Protéine recombinante Ag18435
• Nom complet: N-acetyltransferase 12 (GCN5-related, putative)
• Masse moléculaire calculée: 362 aa, 39 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC118589
• Symbole du gène: NAT12
• Identification du gène (NCBI): 122830
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

N-terminal acetylation (Nt-acetylation) by N-terminal acetyltransferases (NATs) is one of the most common protein modifications in eukaryotes. The NatC complex represents one of three major NATs of which the substrate profile remains largely unexplored. NAT12/NAA30 (N-alpha-acetyltransferase 30) is the catalytic subunit of the NAT-C complex (PMID: 27694331, 26292663). The expected sizes of the two isoforms of the protein were 35 and 39 kDa. Western blots detected two bands of NAA12, the expected ~40 kDa band and a possibly dimer at ~70 kDa.

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