Anticorps Polyclonal de lapin anti-NBR1

• Phare
• Validé par KD/KO

NBR1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

42

N° de cat : 16004-1-AP

Synonymes

1A1 3B, 1A13B, KIAA0049, M17S2, MIG19, NBR1, neighbor of BRCA1 gene 1, Next to BRCA1 gene 1 protein, Protein 1A1 3B

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Galerie de données de validation

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  WB analysis using 16004-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16004-1-AP (NBR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of COS-7 using 16004-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 16004-1-AP (NBR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16004-1-AP

  IP result of anti-NBR1(IP:16004-1-AP, 4ug; Detection:16004-1-AP 1:500) with HeLa cells lysate 2320 ug.

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  IHC staining of mouse heart using 16004-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16004-1-AP (NBR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 16004-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16004-1-AP (NBR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16004-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16004-1-AP (NBR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16004-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16004-1-AP (NBR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16004-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NBR1 antibody (16004-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 16004-1-AP

  1X10^6 HeLa cells were stained with 0.2ug NBR1 antibody (16004-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COS-7
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu cardiaque de souris, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 27 publications below
• IHC: See 10 publications below
• IF: See 14 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

16004-1-AP cible NBR1 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Réactivité citée: canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NBR1 Protéine recombinante Ag8652
• Nom complet: neighbor of BRCA1 gene 1
• Masse moléculaire calculée: 966 aa, 107 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC009808
• Symbole du gène: NBR1
• Identification du gène (NCBI): 4077
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NBR1, also named as 1A13B, KIAA0049 and M17S2, acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets. NBR1 and P62 can bind to autophagic effector proteins (Atg8 in yeast, MAP1LC3 protein family in mammals) anchored in the membrane of autophagosomes. It is a highly conserved multidomain scaffold protein with proposed roles in endocytic trafficking and selective autophagy. NBR1 is a novel PB1 adapter in Th2 differentiation and asthma. It functions as an autophagy receptor involved in targeting ubiquitinated proteins for degradation. It also has a dual role as a scaffold protein to regulate growth-factor receptor and downstream signaling pathways. Observed MW of NBR1 is 140 kDa (PMID: 22654911, PMID: 22484440).

Publications

Species	Application	Title
human	WB	Cell; Lack of Neuronal IFN-β-IFNAR Causes Lewy Body- and Parkinson's Disease-like Dementia.; Authors - Patrick Ejlerskov
mouse	WB, IHC	Cell Metab; Hif-2α promotes degradation of mammalian peroxisomes by selective autophagy.; Authors - Katharina M Walter
human	WB	Autophagy; TOLLIP-mediated autophagic degradation pathway links the VCP-TMEM63A-DERL1 signaling axis to triple-negative breast cancer progression; Authors - Tai-Mei Zhang
human	WB,IF	Autophagy; Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules; Authors - Cuiwei Yang ; KD Validated
human	IF	Autophagy; Identification of a lung cancer cell line deficient in atg7-dependent autophagy.; Authors - Jonathan Mandelbaum
human	IF	Autophagy; Export-deficient monoubiquitinated PEX5 triggers peroxisome removal in SV40 large T antigen-transformed mouse embryonic fibroblasts.; Authors - Marcus Nordgren

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