Anticorps Monoclonal anti-NBR1

NBR1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F2B2

N° de cat : 68062-1-PBS

Synonymes

1A1 3B, 1A13B, 1F2B2, Cell migration-inducing gene 19 protein, FLJ55359

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Galerie de données de validation

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  WB analysis using 68062-1-Ig (same clone as 68062-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68062-1-Ig (NBR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68062-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68062-1-Ig (same clone as 68062-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NBR1 antibody (68062-1-Ig, Clone: 1F2B2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68062-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68062-1-PBS cible NBR1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NBR1 Protéine recombinante Ag8652
• Nom complet: neighbor of BRCA1 gene 1
• Masse moléculaire calculée: 966 aa, 107 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC009808
• Symbole du gène: NBR1
• Identification du gène (NCBI): 4077
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NBR1, also named as 1A13B, KIAA0049 and M17S2, acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets. NBR1 and P62 can bind to autophagic effector proteins (Atg8 in yeast, MAP1LC3 protein family in mammals) anchored in the membrane of autophagosomes. It is a highly conserved multidomain scaffold protein with proposed roles in endocytic trafficking and selective autophagy. NBR1 is a novel PB1 adapter in Th2 differentiation and asthma. It functions as an autophagy receptor involved in targeting ubiquitinated proteins for degradation. It also has a dual role as a scaffold protein to regulate growth-factor receptor and downstream signaling pathways. Observed MW of NBR1 is 140 kDa (PMID: 22654911, PMID: 22484440).

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