Anticorps Monoclonal anti-NCALD

NCALD Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

6B4E10

N° de cat : 66088-1-PBS

Synonymes

NCALD, neurocalcin delta, neurocalcin δ

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Galerie de données de validation

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  WB analysis using 66088-1-Ig (same clone as 66088-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66088-1-Ig (same clone as 66088-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66088-1-Ig (same clone as 66088-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66088-1-Ig (same clone as 66088-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66088-1-Ig (NCALD antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 66088-1-Ig(NCALD antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66088-1-Ig (NCALD Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 66088-1-Ig (NCALD Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66088-1-Ig (NCALD antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cerebellum using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 66088-1-Ig(NCALD antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66088-1-Ig (same clone as 66088-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66088-1-Ig (NCALD antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66088-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66088-1-PBS cible NCALD dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NCALD Protéine recombinante Ag18760
• Nom complet: neurocalcin delta
• Masse moléculaire calculée: 193 aa, 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC036098
• Symbole du gène: NCALD
• Identification du gène (NCBI): 83988
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NCALD(Neurocalcin-delta) is a member of the EF-hand calcium-binding protein superfamily.It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation.It is a high-affinity Ca2+ sensor (Kd of approx. 0.6 uM), which is most abundantly expressed in the Purkinje cells of the cerebellum(PMID:11964161).This antibody is specific to NCALD.

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