Anticorps Monoclonal anti-NCAM1/CD56

NCAM1/CD56 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(9)

CloneNo.

1E8C9

N° de cat : 60238-1-Ig

Synonymes

CD56, NCAM1, 1E8C9, MSK39, N CAM 1

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Galerie de données de validation

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  WB analysis of fetal human brain using 60238-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60238-1-Ig (NCAM1/CD56 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 60238-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 60238-1-Ig (NCAM1/CD56 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 60238-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60238-1-Ig (NCAM1/CD56 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 60238-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 60238-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60238-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 60238-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60238-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 60238-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60238-1-Ig (NCAM1/CD56 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon using 60238-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using NCAM1/CD56 antibody (60238-1-Ig, Clone: 1E8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 60238-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using NCAM1/CD56 antibody (60238-1-Ig, Clone: 1E8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 60238-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using NCAM1/CD56 antibody (60238-1-Ig, Clone: 1E8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, cellules C6, tissu cérébral de porc, tissu cérébral humain
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de côlon humain, tissu d'amygdalite humain
• Résultats positifs en IF/ICC: cellules SH-SY5Y, tissu de côlon humain

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Informations sur le produit

60238-1-Ig cible NCAM1/CD56 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NCAM1/CD56 Protéine recombinante Ag5732
• Nom complet: neural cell adhesion molecule 1
• Masse moléculaire calculée: 95 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC047244
• Symbole du gène: NCAM1
• Identification du gène (NCBI): 4684
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).

Publications

Species	Application	Title
mouse	IHC	Small; Nanobody-Engineered Natural Killer Cell Conjugates for Solid Tumor Adoptive Immunotherapy; Authors - Liang Gong
human	IF	J Transl Med; Systematic analysis of various RNA transcripts and construction of biological regulatory networks at the post-transcriptional level for chronic obstructive pulmonary disease; Authors - Beibei Li
human	IHC	Rheumatology (Oxford); Interleukin-6 trans-signaling regulates monocyte chemoattractant protein-1 production in immune-mediated necrotizing myopathy; Authors - Xue Ma
human	WB	Stem Cells Dev; Effect of the Soluble Factors Released by Dental Apical Papilla-Derived Stem Cells on the Osteo/Odontogenic, Angiogenic, and Neurogenic Differentiation of Dental Pulp Cells.; Authors - Shi Yu
human	IHC	Jpn J Clin Oncol; Identification of distinct genomic features reveals frequent somatic AHNAK and PTEN mutations predominantly in primary malignant melanoma presenting in the ureter.; Authors - Yan Huang
mouse,human	IF	Hepatology; Hepatocyte-derived MASP1-enriched small extracellular vesicles activate HSCs to promote liver fibrosis; Authors - Xianzhi Liu

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