Anticorps Polyclonal de lapin anti-NCAM1/CD56

• Phare
• Validé par KD/KO

NCAM1/CD56 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 14255-1-PBS

Synonymes

CD56, NCAM1, MSK39, N CAM 1, NCAM

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Galerie de données de validation

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  WB analysis of Neuro-2a using 14255-1-AP (same clone as 14255-1-PBS)

  WB result of NCAM1/CD56 antibody (14255-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAM1/CD56 transfected Neuro-2a cells. This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 14255-1-AP (same clone as 14255-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14255-1-AP (NCAM1/CD56 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  WB analysis using 14255-1-AP (same clone as 14255-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:16000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:16000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small cell lung carcinoma tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:16000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small cell lung carcinoma slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:16000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  IHC staining of Insulinoma using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of Insulinoma using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Insulinoma tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:16000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:16000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:64000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:64000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 14255-1-AP (same clone as 14255-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14255-1-AP (NCAM1/CD56 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 14255-1-AP (same clone as 14255-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using NCAM1/CD56 antibody (14255-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14255-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14255-1-PBS cible NCAM1/CD56 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NCAM1/CD56 Protéine recombinante Ag5528
• Nom complet: neural cell adhesion molecule 1
• Masse moléculaire calculée: 95 kDa
• Poids moléculaire observé: 120 kDa, 140 kDa, 180 kDa
• Numéro d’acquisition GenBank: BC047244
• Symbole du gène: NCAM1
• Identification du gène (NCBI): 4684
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).

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