Anticorps Polyclonal de lapin anti-NCAM1/CD56

NCAM1/CD56 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 31536-1-AP

Synonymes

CD56, Ncam1, NCAM 1, Ncam, E NCAM

---

Galerie de données de validation

• 

  WB analysis using 31536-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31536-1-AP (NCAM1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 31536-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31536-1-AP (NCAM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 31536-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31536-1-AP (NCAM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 31536-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31536-1-AP (NCAM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 31536-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31536-1-AP (NCAM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu de cervelet de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

31536-1-AP cible NCAM1/CD56 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: neural cell adhesion molecule 1
• Masse moléculaire calculée: 119 kDa
• Poids moléculaire observé: 120 kDa, 140 kDa, 180 kDa
• Symbole du gène: Ncam1
• Identification du gène (NCBI): 17967
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).

{{ptg:RelatedPrimaryAntibodies}}