Anticorps Recombinant de lapin anti-NCAM1/CD56

NCAM1/CD56 Recombinant Antibody for IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5N5

N° de cat : 82715-3-PBS

Synonymes

CD56, NCAM1, 5N5, MSK39, N CAM 1

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Galerie de données de validation

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  IHC staining of human tonsillitis using 82715-3-RR (same clone as 82715-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82715-3-RR (NCAM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82715-3-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 82715-3-RR (same clone as 82715-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82715-3-RR (NCAM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82715-3-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82715-3-RR (same clone as 82715-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82715-3-RR (NCAM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82715-3-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82715-3-RR (same clone as 82715-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82715-3-RR (NCAM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82715-3-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 82715-3-RR (same clone as 82715-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using NCAM1/CD56 antibody (82715-3-RR, Clone: 5N5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82715-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82715-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 82715-3-PBS against Human NCAM1/CD56 was performed. The affinity constant is 0.795 nM.

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  Affinity and Kinetic Characterization of 82715-3-RR

  Biolayer interferometry (BLl) kinetic assays of 82715-3-RR against Human NCAM1/CD56 were performed. The affinity constant is 1.45 nM.

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Informations sur le produit

82715-3-PBS cible NCAM1/CD56 dans les applications de IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NCAM1/CD56 Protéine recombinante Ag5528
• Nom complet: neural cell adhesion molecule 1
• Masse moléculaire calculée: 95 kDa
• Numéro d’acquisition GenBank: BC047244
• Symbole du gène: NCAM1
• Identification du gène (NCBI): 4684
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isoforms have also been described (PMID: 1856291).

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