Anticorps Monoclonal anti-NCAM1/CD56
NCAM1/CD56 Monoclonal Antibody for IF/ICC
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Humain, porc, rat, souris
Applications
IF/ICC
Conjugaison
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1E8C9
N° de cat : CL488-60238
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en IF/ICC | cellules SH-SY5Y, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Informations sur le produit
CL488-60238 cible NCAM1/CD56 dans les applications de IF/ICC et montre une réactivité avec des échantillons Humain, porc, rat, souris
| Réactivité | Humain, porc, rat, souris |
| Hôte / Isotype | Mouse / IgG1 |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | NCAM1/CD56 Protéine recombinante Ag5732 |
| Nom complet | neural cell adhesion molecule 1 |
| Masse moléculaire calculée | 95 kDa |
| Poids moléculaire observé | 140 kDa |
| Numéro d’acquisition GenBank | BC047244 |
| Symbole du gène | NCAM1 |
| Identification du gène (NCBI) | 4684 |
| Conjugaison | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Forme | Liquide |
| Méthode de purification | Purification par protéine G |
| Tampon de stockage | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
| Conditions de stockage | Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).
Protocole
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 NCAM1/CD56 antibody CL488-60238 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
