Anticorps Monoclonal anti-NCAM1/CD56

NCAM1/CD56 Monoclonal Antibody for IF/ICC

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

IF/ICC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E8C9

N° de cat : CL488-60238

Synonymes

CD56, NCAM1, 1E8C9, NCAM 1, NCAM



Applications testées

Résultats positifs en IF/ICCcellules SH-SY5Y,

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-60238 cible NCAM1/CD56 dans les applications de IF/ICC et montre une réactivité avec des échantillons Humain, porc, rat, souris

Réactivité Humain, porc, rat, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène NCAM1/CD56 Protéine recombinante Ag5732
Nom complet neural cell adhesion molecule 1
Masse moléculaire calculée 95 kDa
Poids moléculaire observé 140 kDa
Numéro d’acquisition GenBankBC047244
Symbole du gène NCAM1
Identification du gène (NCBI) 4684
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519). Several other isofroms have also been described (PMID: 1856291).

Protocole

Product Specific Protocols
IF protocol for CL Plus 488 NCAM1/CD56 antibody CL488-60238Download protocol
Standard Protocols
Click here to view our Standard Protocols
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