Anticorps Recombinant de lapin anti-NCAM2

NCAM2 Recombinant Antibody for WB, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

240398D9

N° de cat : 83494-6-RR

Synonymes

NCAM21, NCAM-2, NCAM 2, N CAM 2, 240398D9

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Galerie de données de validation

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  WB analysis using 83494-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83494-6-RR (NCAM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using 83494-6-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NCAM2 antibody (83494-6-RR, Clone: 240398D9 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83494-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83494-6-RR against Human NCAM2 were performed. The affinity constant is 4.48 nM.

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Applications testées

• Résultats positifs en WB: cellules LNCaP, tissu cérébral de souris
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83494-6-RR cible NCAM2 dans les applications de WB, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NCAM2 Protéine recombinante Ag4821
• Nom complet: neural cell adhesion molecule 2
• Masse moléculaire calculée: 93 kDa
• Poids moléculaire observé: 125 kDa
• Numéro d’acquisition GenBank: BC052946
• Symbole du gène: NCAM2
• Identification du gène (NCBI): 4685
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The mammalian NCAM family has two members, NCAM1 and NCAM2 (PMID: 9729879; 20439718). Neural cell adhesion molecule 2 (NCAM2, also known as NCAM21) is a single-pass type I membrane protein that is highly expressed in the brain, which may play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons or in neurodegenerative diseases such as Alzheimer's disease (PMID: 26611261). NCAM2 has two isoforms: NCAM2.1 bearing transmembrane and cytoplasmic domains and the shorter NCAM2.2 isoform, which is GPI-anchored (PMID: 9334170; 9221781). The molecular weight of NCAM2 is around 125 kDa and 93 kDa (PMID: 9334170; 3171685).

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