Anticorps Polyclonal de lapin anti-NCAPD2

• Phare
• Validé par KD/KO

NCAPD2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13382-1-PBS

Synonymes

Condensin complex subunit 1, CNAP1, Chromosome-associated protein D2, Chromosome condensation-related SMC-associated protein 1, CAPD2

---

Galerie de données de validation

• 

  WB analysis using 13382-1-AP (same clone as 13382-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 13382-1-AP (same clone as 13382-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 13382-1-AP (same clone as 13382-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 13382-1-AP (same clone as 13382-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 13382-1-AP (same clone as 13382-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 13382-1-AP (NCAPD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 13382-1-AP (same clone as 13382-1-PBS)

  IP result of anti-NCAPD2 (IP:13382-1-AP, 4ug; Detection:13382-1-AP 1:600) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 13382-1-AP (same clone as 13382-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13382-1-AP (NCAPD2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 13382-1-AP (same clone as 13382-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 13382-1-AP (NCAPD2 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 13382-1-AP (same clone as 13382-1-PBS)

  Immunofluorescent analysis of Hela cells, using NCAPD2 antibody 13382-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 13382-1-AP (same clone as 13382-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13382-1-AP (NCAPD2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13382-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

13382-1-PBS cible NCAPD2 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NCAPD2 Protéine recombinante Ag4219
• Nom complet: non-SMC condensin I complex, subunit D2
• Masse moléculaire calculée: 1401 aa, 157 kDa
• Poids moléculaire observé: 157 kDa
• Numéro d’acquisition GenBank: BC028182
• Symbole du gène: NCAPD2
• Identification du gène (NCBI): 9918
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NCAPD2 is a regulatory subunit of the condensin complex, which is required for conversion of interphase chromatin into mitotic-like condense chromosomes.  Condensin complexes are heteropentamers comprised of two SMC (structural maintenance of chromosomes) subunits and three non-SMC subunits. The C-terminus of NCAPD2 interacts with Histones H1 and H3 through their histone tails. A loss of NCAPD2 can lead to the disorganization of chromatid axes, misalignment of sister chromatids during metaphase and delayed entry into anaphase.

{{ptg:RelatedPrimaryAntibodies}}