Anticorps Polyclonal de lapin anti-NCAPD3

NCAPD3 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 16828-1-AP

Synonymes

CAP D3, CAPD3, CNDD3, Condensin 2 complex subunit D3, hCAP D3, hCAP-D3, hcp 6, hHCP 6, KIAA0056, NCAPD3

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Galerie de données de validation

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  WB analysis of HepG2 using 16828-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 16828-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16828-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16828-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16828-1-AP

  IP Result of anti-NCAPD3 (IP:16828-1-AP, 4ug; Detection:16828-1-AP 1:500) with HeLa cells lysate 600ug.

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  IF Staining of HepG2 using 16828-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16828-1-AP (NCAPD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, cellules MCF-7
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

16828-1-AP cible NCAPD3 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: bovin, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NCAPD3 Protéine recombinante Ag10429
• Nom complet: non-SMC condensin II complex, subunit D3
• Masse moléculaire calculée: 1498 aa, 169 kDa
• Poids moléculaire observé: 165 kDa
• Numéro d’acquisition GenBank: BC011408
• Symbole du gène: NCAPD3
• Identification du gène (NCBI): 23310
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 and SMC4 , but they contain different sets of non-SMC subunits. NCAPD3 is 1 of 3 non-SMC subunits that define condensin II ([PMID: 14532007).

Publications

Species	Application	Title
	WB	Elife; Molecular dissection of condensin II-mediated chromosome assembly using in vitro assays; Authors - Makoto M Yoshida
bovine	IF	Int J Mol Sci; NCAPG Dynamically Coordinates the Myogenesis of Fetal Bovine Tissue by Adjusting Chromatin Accessibility.; Authors - Xin Hu
human	WB	World J Gastroenterol; Non-SMC condensin I complex subunit D2 and non-SMC condensin II complex subunit D3 induces inflammation via the IKK/NF-κB pathway in ulcerative colitis.; Authors - Chang-Wen Yuan
human	WB	Mol Cells; NCAPH Stabilizes GEN1 in Chromatin to Resolve Ultra-Fine DNA Bridges and Maintain Chromosome Stability; Authors - Jae Hyeong Kim
	WB	Mol Biol Cell; Regulation of condensin II by self-suppression and release mechanisms; Authors - Makoto M Yoshida