Anticorps Polyclonal de lapin anti-NCAPD3

• Phare
• Validé par KD/KO

NCAPD3 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16828-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HepG2 using 16828-1-AP (same clone as 16828-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

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  WB analysis using 16828-1-AP (same clone as 16828-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16828-1-AP (same clone as 16828-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16828-1-AP (same clone as 16828-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16828-1-AP (NCAPD3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 16828-1-AP (same clone as 16828-1-PBS)

  IP result of anti-NCAPD3 (IP:16828-1-AP, 4ug; Detection:16828-1-AP 1:500) with HeLa cells lysate 600ug. This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 16828-1-AP (same clone as 16828-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 16828-1-AP (NCAPD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16828-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16828-1-PBS cible NCAPD3 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NCAPD3 Protéine recombinante Ag10429
• Nom complet: non-SMC condensin II complex, subunit D3
• Masse moléculaire calculée: 1498 aa, 169 kDa
• Poids moléculaire observé: 165 kDa
• Numéro d’acquisition GenBank: BC011408
• Symbole du gène: NCAPD3
• Identification du gène (NCBI): 23310
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Condensin complexes I and II play essential roles in mitotic chromosome assembly and segregation. Both condensins contain 2 invariant structural maintenance of chromosome (SMC) subunits, SMC2 and SMC4 , but they contain different sets of non-SMC subunits. NCAPD3 is 1 of 3 non-SMC subunits that define condensin II ([PMID: 14532007).

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