Anticorps Monoclonal anti-NCAPG

NCAPG Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D4D3

N° de cat : 68082-1-PBS

Synonymes

CAPG, CHCG, Condensin complex subunit 3, Condensin subunit CAP G, FLJ12450, HCAP G, Melanoma antigen NY MEL 3, NCAPG, NY MEL 3, NYMEL3, XCAP G homolog

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Galerie de données de validation

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  WB analysis using 68082-1-Ig (same clone as 68082-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68082-1-Ig (NCAPG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68082-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 68082-1-Ig (same clone as 68082-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NCAPG antibody (68082-1-Ig, Clone: 2D4D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68082-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 68082-1-Ig (same clone as 68082-1-PBS)

  1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human NCAPG (68082-1-Ig, Clone:2D4D3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68082-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68082-1-PBS cible NCAPG dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NCAPG Protéine recombinante Ag19926
• Nom complet: non-SMC condensin I complex, subunit G
• Masse moléculaire calculée: 1015 aa, 114 kDa
• Poids moléculaire observé: 114 kDa
• Numéro d’acquisition GenBank: BC068467
• Symbole du gène: NCAPG
• Identification du gène (NCBI): 64151
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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