Anticorps Polyclonal de lapin anti-NOXA2/p67phox

NOXA2/p67phox Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(26)

N° de cat : 15551-1-AP

Synonymes

NCF2, NOXA2, P67PHOX, NCF 2, NCF-2

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Galerie de données de validation

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  WB analysis using 15551-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 15551-1-AP (NOXA2/p67phox antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15551-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15551-1-AP (NOXA2/p67phox antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 15551-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15551-1-AP (NOXA2/p67phox antibody at dilution of 1:50(under 10x lens).

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  IHC staining of human tonsillitis using 15551-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15551-1-AP (NOXA2/p67phox antibody at dilution of 1:50(under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules U-251, cellules COLO 320, cellules MCF-7
• Résultats positifs en IHC: tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 21 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

15551-1-AP cible NOXA2/p67phox dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NOXA2/p67phox Protéine recombinante Ag7909
• Nom complet: neutrophil cytosolic factor 2
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 67 kDa
• Numéro d’acquisition GenBank: BC001606
• Symbole du gène: NCF2
• Identification du gène (NCBI): 4688
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NOXA2/p67phox is also named as NCF2(Neutrophil cytosol factor 2) and belongs to the NCF2/NOXA1 family. It is a membrane bound cytochrome b558 required for activation of the latent NADPH oxidase. NCF2 is an essential component of the multi-protein NADPH oxidase enzyme in phagocytic leukocytes, as well as in certain non-phagocytic cells. This protein has 4 isoforms produced by alternative splicing.

Publications

Species	Application	Title
rat	WB	ACS Nano; Oxidation of Reduced Graphene Oxide via Cellular Redox Signaling Modulates Actin-Mediated Neurotransmission.; Authors - Yiyuan Kang
human	WB	J Hazard Mater; Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells.; Authors - Feng Xiaoli
human	WB	Redox Biol; Serum response factor (SRF) promotes ROS generation and hepatic stellate cell activation by epigenetically stimulating NCF1/2 transcription.; Authors - Ming Kong
mouse	WB,IF	Br J Pharmacol; Rhein regulates redox-mediated activation of NLRP3 inflammasomes in intestinal inflammation through macrophage-activated crosstalk.; Authors - Yangyang Zhou
human	WB	Free Radic Biol Med; S100A8 induces Cyclophosphamide-Induced Alopecia via NCF2/NOX2-Mediated Ferroptosis; Authors - Wen Xu
rat	WB	Oxid Med Cell Longev; Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates.; Authors - Mengyang Zhang

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