Anticorps Polyclonal de lapin anti-NCKAP1

NCKAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12140-1-PBS

Synonymes

NAP125, NAP1, NAP 1, Membrane-associated protein HEM-2, KIAA0587

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Galerie de données de validation

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  WB analysis of mouse brain using 12140-1-AP (same clone as 12140-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12140-1-AP (NCKAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

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  WB analysis using 12140-1-AP (same clone as 12140-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12140-1-AP (NCKAP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12140-1-AP (same clone as 12140-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12140-1-AP (NAP1;NCKAP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 12140-1-AP (same clone as 12140-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12140-1-AP (NAP1;NCKAP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12140-1-AP (same clone as 12140-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12140-1-AP (NAP1;NCKAP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 12140-1-AP (same clone as 12140-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12140-1-AP (NAP1;NCKAP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 12140-1-AP (same clone as 12140-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCKAP1 antibody (12140-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 12140-1-AP (same clone as 12140-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using NCKAP1 antibody (12140-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12140-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12140-1-PBS cible NCKAP1 dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NCKAP1 Protéine recombinante Ag2788
• Nom complet: NCK-associated protein 1
• Masse moléculaire calculée: 129 kDa
• Poids moléculaire observé: 115-125 kDa
• Numéro d’acquisition GenBank: BC015025
• Symbole du gène: NCKAP1
• Identification du gène (NCBI): 10787
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NCK-associated protein 1 (NCKAP1), also known as NAP1 or HEM2, is encoded by an apoptosis-related gene human NAP1, the expression of which is strongly down-regulated in sporadic Alzheimer's disease (AD). Human NAP1 is predominantly expressed in neuronal cells. Antisense oligo DNA of human NAP1 transcripts was found to induce apoptosis of neuronal cells. Loss of NAP1 function disrupts neuronal differentiation. Likewise, NAP1 plays an essential role in facilitating neuronal cytoskeletal changes underlying the postmigratory differentiation of cortical neurons, a critical step in functional wiring of the cortex. Human NAP1 is also proved to be an orthologue of rat Nap1 which binds to the adaptor molecule NCK in signal transduction.

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