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Anticorps Polyclonal de lapin anti-NCKAP5

NCKAP5 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31199-1-PBS

Synonymes

NAP5, Peripheral clock protein, Nck associated protein 5, NAP 5, ERIH2

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using NCKAP5 Polyclonal antibody (31199-1-AP)

    WB analysis using 31199-1-AP (same clone as 31199-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31199-1-AP (NCKAP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31199-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NCKAP5 Polyclonal antibody (31199-1-AP)

    WB analysis using 31199-1-AP (same clone as 31199-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31199-1-AP (NCKAP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31199-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NCKAP5 Polyclonal antibody (31199-1-AP)

    IF Staining of U2OS using 31199-1-AP (same clone as 31199-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NCKAP5 antibody (31199-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31199-1-PBS in a different storage buffer formulation.

Informations sur le produit

31199-1-PBS cible NCKAP5 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène NCKAP5 Protéine recombinante Ag35092
Nom complet Nck-associated protein 5
Masse moléculaire calculée209 kDa
Poids moléculaire observé250-260 kDa
Numéro d’acquisition GenBankNM_207363
Symbole du gène NAP5
Identification du gène (NCBI) 344148
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NCKAP5 (Nck-associated protein 5), also known as NAP5. NCKAP5 is predicted to be located in cells, especially in nucleoli, Golgi apparatus, cell membrane and cytoplasm. It is widely expressed in many tissues, especially in lung and kidney, and in more than 20 other tissues. And the expression of NCKAP5 was enhanced in oligodendrocytes, oligodendrocyte precursor cells and astrocytes. It may interact with other protein, such as NPM1 and centromere protein complex, and regulate accurate chromosome segregation and distribution. NCKAP5 may be involved in the formation and development of tumors, especially in the process of tumor variation such as ovary and breast cancer. In addition, NCKAP5 may be a diagnostic biomarker in non-small cell lung cancer (NSCLC), which is related to the infiltration of activated B cells, regulatory T cells (Tregs), neutrophils and dendritic cells (PMID: 36628881). The molecular weight of NCKAP5 is 208 kDa, it also has some protein modifications.

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