Anticorps Polyclonal de lapin anti-NCOA1/SRC-1

NCOA1/SRC-1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21952-1-PBS

Synonymes

NCOA1, SRC 1, bHLHe42, BHLHE74, Class E basic helix-loop-helix protein 74

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Galerie de données de validation

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  WB analysis using 21952-1-AP (same clone as 21952-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21952-1-AP (NCOA1/SRC-1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21952-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21952-1-AP (same clone as 21952-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21952-1-AP (NCOA1/SRC-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21952-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21952-1-PBS cible NCOA1/SRC-1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NCOA1/SRC-1 Protéine recombinante Ag16603
• Nom complet: nuclear receptor coactivator 1
• Masse moléculaire calculée: 1441 aa, 157 kDa
• Poids moléculaire observé: 140-160 kDa
• Numéro d’acquisition GenBank: BC111533
• Symbole du gène: SRC 1/NCOA1
• Identification du gène (NCBI): 8648
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear receptor coactivator 1, also named SRC-1, directly binds nuclear receptors and stimulates transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR, and ER), retinoids (RXRs), thyroid hormone (TRs), and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B, and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via the remodeling of chromatin, and possibly act by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.

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