Anticorps Polyclonal de lapin anti-NCOA5

• Phare
• Validé par KD/KO

NCOA5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20175-1-PBS

Synonymes

Nuclear receptor coactivator 5, NCoA-5, KIAA1637, Coactivator independent of AF-2, CIA

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Galerie de données de validation

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  WB analysis using 20175-1-AP (same clone as 20175-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20175-1-AP (NCOA5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20175-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 20175-1-AP (same clone as 20175-1-PBS)

  IP result of anti-NCOA5 (IP:20175-1-AP, 4ug; Detection:20175-1-AP 1:800) with HEK-293 cells lysate 3000ug. This data was developed using the same antibody clone with 20175-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 20175-1-AP (same clone as 20175-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 20175-1-AP (NCOA5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20175-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 20175-1-AP (same clone as 20175-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using NCOA5 antibody (20175-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 20175-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 20175-1-AP (same clone as 20175-1-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug NCOA5 Polyclonal antibody (20175-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 20175-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20175-1-PBS cible NCOA5 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NCOA5 Protéine recombinante Ag14014
• Nom complet: nuclear receptor coactivator 5
• Masse moléculaire calculée: 579 aa, 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC056872
• Symbole du gène: NCOA5
• Identification du gène (NCBI): 57727
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear receptor coactivator 5 (NCOA5), also known as coactivator independent of AF-2 (CIA), possesses both repressor and activator functions (PMID: 11113208). It can interact with Estrogen Receptor (ER) alpha and ER beta in a ligand-dependent manner. NCOA5 has been shown to regulate the ER alpha-mediated transcription as well as the expression of the c-Myc gene (PMID: 11113208; 15073177). Recently identified as a conserved component of pluripotent stem cells, mammalian NCOA5 may contribute to the maintenance of the pluripotent stem cell population (PMID: 24268775).

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