Anticorps Polyclonal de lapin anti-NCOA7

• Phare
• Validé par KD/KO

NCOA7 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 23092-1-AP

Synonymes

ERAP140, ESNA1, Nbla00052, Nbla10993, NCOA7, nuclear receptor coactivator 7

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Galerie de données de validation

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  WB analysis of HEK-293 using 23092-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23092-1-AP (NCOA7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23092-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23092-1-AP (NCOA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 23092-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23092-1-AP (NCOA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 23092-1-AP

  IP Result of anti-NCOA7 (IP:23092-1-AP, 4ug; Detection:23092-1-AP 1:1000) with HEK-293 cells lysate 3600ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules MCF-7
• Résultats positifs en IP: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

23092-1-AP cible NCOA7 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NCOA7 Protéine recombinante Ag19315
• Nom complet: nuclear receptor coactivator 7
• Masse moléculaire calculée: 972 aa, 106 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: BC137094
• Symbole du gène: NCOA7
• Identification du gène (NCBI): 135112
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear receptors for steroids, thyroid hormones and retinoic acids are ligand-dependent transcription factors that activate transcription through specific DNA binding sites in their target genes. NCoA-7 (nuclear receptor coactivator 7), also known as ESNA1 or ERAP140, is a 942 amino acid nuclear protein that enhances nuclear receptor transcriptional activities and coactivates several nuclear receptors including PPARG, ESR1, THRB and RARA. Highly expressed in brain and weakly expressed in pancreas, bladder, ovary, spinal cord, prostate, mammary gland, ovary, uterus and stomach, NCoA-7 is a member of the OXR1 family and contains one LysM repeat and a TLD domain. This antibody is a rabbit polyclonal antibody raised against the N-terminal of human NCOA7 protein.

Publications

Species	Application	Title
mouse	WB,CoIP	Cell Mol Life Sci; The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour.; Authors - Enrico Castroflorio ; KO Validated
		Am J Physiol Renal Physiol; Targeted Deletion of the Ncoa7 Gene Results in Incomplete Distal Renal Tubular Acidosis in Mice.; Authors - Maria Merkulova
human	WB,IHC,IF	Int J Mol Sci; NCOA7 Regulates Growth and Metastasis of Clear Cell Renal Cell Carcinoma via MAPK/ERK Signaling Pathway; Authors - Jiayu Guo