Anticorps Polyclonal de lapin anti-NCOR2

NCOR2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29952-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 29952-1-AP (same clone as 29952-1-PBS)

  MOLT-4 cells were subjected to Tris-Acetate gel system followed by western blot with 29952-1-AP (NCOR2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29952-1-PBS in a different storage buffer formulation.

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  WB analysis using 29952-1-AP (same clone as 29952-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29952-1-AP (NCOR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29952-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 29952-1-AP (same clone as 29952-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29952-1-AP (NCOR2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29952-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 29952-1-AP (same clone as 29952-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29952-1-AP (NCOR2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29952-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 29952-1-AP (same clone as 29952-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using NCOR2 antibody (29952-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29952-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29952-1-PBS cible NCOR2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NCOR2 Protéine recombinante Ag30727
• Nom complet: nuclear receptor co-repressor 2
• Masse moléculaire calculée: 274 kDa
• Poids moléculaire observé: 274 kDa
• Numéro d’acquisition GenBank: NM_006312
• Symbole du gène: NCOR2
• Identification du gène (NCBI): 9612
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NCOR2, nuclear receptor corepressor 2 , is a transcriptional corepressor that that contains several nuclear receptor-interacting domains. NCOR2/SMRT acts as a platform protein, facilitating the recruitment of histone deacetylases to the DNA promoters bound by its interacting transcription factors (PMID: 9150137). NCOR2 mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription (PMID: 23081895).

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