Anticorps Monoclonal anti-NCS1

NCS1 Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F10C8

N° de cat : 67616-1-PBS

Synonymes

FREQ, FLUP, Frequenin-like protein, Frequenin like protein, frequenin homolog (Drosophila)

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Galerie de données de validation

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  WB analysis using 67616-1-Ig (same clone as 67616-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67616-1-Ig (NCS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67616-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67616-1-Ig (same clone as 67616-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67616-1-Ig (NCS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67616-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67616-1-Ig (same clone as 67616-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67616-1-Ig (NCS1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67616-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 67616-1-Ig (same clone as 67616-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCS1 antibody (67616-1-Ig, Clone: 2F10C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67616-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51282-1

  Cytometric bead array standard curve of MP51282-1, FREQ Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67616-1-PBS. Detection antibody: 67616-2-PBS. Standard:Ag29808. Range: 1.563-100 ng/mL.

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Informations sur le produit

67616-1-PBS cible NCS1 dans les applications de WB, IHC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NCS1 Protéine recombinante Ag29808
• Nom complet: frequenin homolog (Drosophila)
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC004856
• Symbole du gène: NCS1
• Identification du gène (NCBI): 23413
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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