Anticorps Polyclonal de lapin anti-NCX1

NCX1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28447-1-PBS

Synonymes

Na(+)/Ca(2+)-exchange protein 1, SLC8A1, Solute carrier family 8 member 1

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Galerie de données de validation

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  WB analysis using 28447-1-AP (same clone as 28447-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28447-1-AP (NCX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28447-1-PBS in a different storage buffer formulation.

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  WB analysis using 28447-1-AP (same clone as 28447-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28447-1-AP (NCX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28447-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 28447-1-AP (same clone as 28447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28447-1-AP (NCX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28447-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 28447-1-AP (same clone as 28447-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28447-1-AP (NCX1 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28447-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28447-1-PBS cible NCX1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NCX1 Protéine recombinante Ag28952
• Nom complet: solute carrier family 8 (sodium/calcium exchanger), member 1
• Masse moléculaire calculée: 109 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: NM_021097
• Symbole du gène: NCX1
• Identification du gène (NCBI): 6546
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SLC8A1, also known as NCX1, belongs to the SLC (Solute Carrier) family and the CaCA (Calcium/Cation Antiporter) superfamily. NCX1 is a bidirectional ion exchanger that can exchange sodium and calcium ions between the inside and outside of cells in a 1:3 ratio (1 Ca2+ ion and 3 Na+ ions). Under physiological conditions, NCX1 mainly transports Ca2+ from the cell interior to the exterior through the forward transport mode to maintain the low calcium level inside the cell. However, under certain special conditions, such as ischemia-reperfusion injury and stroke, the reverse transport mode of NCX1 is activated, leading to the influx of Ca2+ into the cell, which may cause cell damage (PMID: 21289289, 30686898).

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