Anticorps Polyclonal de lapin anti-ND2

• Phare
• Validé par KD/KO

ND2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(37)

Avis (1)

N° de cat : 19704-1-AP

Synonymes

MT-ND2, ND 2, NADH2, NADH dehydrogenase subunit 2, MTND2

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Galerie de données de validation

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  WB analysis using 19704-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19704-1-AP (ND2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 19704-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 19704-1-AP (ND2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 19704-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19704-1-AP (ND2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris,
• Résultats positifs en IHC: tissu d'estomac humain, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 37 publications below
• IHC: See 1 publications below

Informations sur le produit

19704-1-AP cible ND2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: NADH dehydrogenase, subunit 2 (complex I)
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 42-44 kDa
• Numéro d’acquisition GenBank: GQ398480
• Symbole du gène: ND2
• Identification du gène (NCBI): 4536
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ND2, also named as MTND2, NADH2 and ND2, belongs to the complex I subunit 2 family. ND2 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Publications

Species	Application	Title
human	WB	Nat Med; The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity.; Authors - Wenzhang Wang
human	WB	Mol Cell; Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis; Authors - Yi Fu
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang ; KD Validated
human	WB	Nat Commun; CO2-sensitive tRNA modification associated with human mitochondrial disease.; Authors - Huan Lin
human	WB	Nat Chem Biol; NSUN3 methylase initiates 5-formylcytidine biogenesis in human mitochondrial tRNA(Met).; Authors - Saori Nakano
human	WB	Nucleic Acids Res; Hypertension-associated mitochondrial DNA 4401A>G mutation caused the aberrant processing of tRNAMet, all 8 tRNAs and ND6 mRNA in the light-strand transcript.; Authors - Xiaoxu Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mi (Verified Customer) (02-01-2023)

works very well in human adipocyte cell lines

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: adipocytes

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