Anticorps Recombinant de lapin anti-ND4

ND4 Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

242422E2

N° de cat : 85098-1-PBS

Synonymes

MT-ND4, 242422E2, MT ND4, MTND4, NADH4

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Galerie de données de validation

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  WB analysis using 85098-1-RR (same clone as 85098-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85098-1-RR (ND4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85098-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02094-1

  Sandwich ELISA standard curve of MP02094-1, Human ND4 Recombinant Matched Antibody Pair - PBS only. 85098-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY01674. 85098-2-PBS was HRP conjugated as the detection antibody. Range: 12.5-800 pg/mL.

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  Sample test of MP02094-1

  The mean ND4 concentration was determined to be 23.9 ng/mL in mouse liver tissue extract based on a 3.7 mg/mL extract load.

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  IHC staining of human stomach cancer using 85098-1-RR (same clone as 85098-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 85098-1-RR (ND4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85098-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 85098-1-RR (same clone as 85098-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 85098-1-RR (ND4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85098-1-PBS in a different storage buffer formulation.

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Informations sur le produit

85098-1-PBS cible ND4 dans les applications de WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: NADH dehydrogenase, subunit 4 (complex I)
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 43-52 kDa
• Numéro d’acquisition GenBank: YP_003024035
• Symbole du gène: ND4
• Identification du gène (NCBI): 4538
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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