Anticorps Polyclonal de lapin anti-MT-ND5

MT-ND5 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(36)

Avis (1)

N° de cat : 55410-1-AP

Synonymes

ND5, ND 5, NADH5, MTND5, MT ND5

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Galerie de données de validation

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  WB analysis using 55410-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55410-1-AP (ND5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 55410-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55410-1-AP (MT-ND5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 55410-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55410-1-AP (ND5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 55410-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55410-1-AP (ND5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique de rat
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 36 publications below

Informations sur le produit

55410-1-AP cible MT-ND5 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: NADH dehydrogenase, subunit 5 (complex I)
• Masse moléculaire calculée: 67 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: AF465942
• Symbole du gène: MT-ND5
• Identification du gène (NCBI): 4540
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ND5 belongs to the complex I subunit 1 family. ND5 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Publications

Species	Application	Title
human	WB	Cell Metab; Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.; Authors - Yi-Ping Wang
human	WB	Mol Cell; Hepatic micropeptide modulates mitochondrial RNA processing machinery in hepatocellular carcinoma; Authors - Linyu Zhu
human	WB	Mol Cell; Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.; Authors - Bin Jiang
human	WB	Sci Adv; MCART1/SLC25A51 is required for mitochondrial NAD transport.; Authors - Nora Kory
human	WB	EMBO J; EglN2 associates with the NRF1-PGC1α complex and controls mitochondrial function in breast cancer.; Authors - Jing Zhang
human	WB	EMBO Rep; MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex.; Authors - Cong Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Baptiste (Verified Customer) (05-23-2025)

Working well but have a light unspecific (?) band close to ND5.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Lymphoblastoid

MT-ND5 Antibody Western Blot validation (1:500 dilution) in Lymphoblastoid (Cat no:55410-1-AP)

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