Anticorps Polyclonal de lapin anti-NDC80

NDC80 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 18932-1-AP

Synonymes

Kinetochore-associated protein 2, Kinetochore protein Hec1, hsNDC80, HsHec1, HEC1

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Galerie de données de validation

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  WB analysis using 18932-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18932-1-AP (NDC80 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 18932-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 18932-1-AP (NDC80 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 18932-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 18932-1-AP (NDC80 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 18932-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 18932-1-AP (NDC80 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A2780 using 18932-1-AP

  IP result of anti-NDC80 (IP:18932-1-AP, 4ug; Detection:18932-1-AP 1:500) with A2780 cells lysate 960ug.

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  IHC staining of human brain using 18932-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18932-1-AP (NDC80 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 18932-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18932-1-AP (NDC80 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A549 using 18932-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using NDC80 antibody (18932-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A2780, cellules Jurkat, tissu splénique humain, tissu testiculaire de souris
• Résultats positifs en IP: cellules A2780
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

18932-1-AP cible NDC80 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDC80 Protéine recombinante Ag8065
• Nom complet: NDC80 homolog, kinetochore complex component (S. cerevisiae)
• Masse moléculaire calculée: 74 kDa
• Poids moléculaire observé: 76 kDa
• Numéro d’acquisition GenBank: BC035617
• Symbole du gène: NDC80
• Identification du gène (NCBI): 10403
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IP	J Cell Biol; PP6 regulation of Aurora A-TPX2 limits NDC80 phosphorylation and mitotic spindle size; Authors - Tomoaki Sobajima
human	WB	Oncotarget; Co-expression of mitosis-regulating genes contributes to malignant progression and prognosis in oligodendrogliomas.; Authors - Yanwei Liu
human	WB,IHC	Front Pharmacol; Upregulation of nuclear division cycle 80 contributes to therapeutic resistance via the promotion of autophagy-related protein-7-dependent autophagy in lung cancer; Authors - Xi Chen ; KD Validated
human	WB	Onco Targets Ther; Targeting DTL induces cell cycle arrest and senescence and suppresses cell growth and colony formation through TPX2 inhibition in human hepatocellular carcinoma cells.; Authors - Yu-Chia Chen
	IHC	iScience; NDC80 status pinpoints mitotic kinase inhibitors as emerging therapeutic options in clear cell renal cell carcinoma; Authors - Cheng Hu
human	WB,IF	CNS Neurosci Ther; NDC80/HEC1 promotes macrophage polarization and predicts glioma prognosis via single-cell RNA-seq and in vitro experiment; Authors - Weijie Ye ; KD Validated

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