Anticorps Polyclonal de lapin anti-NDFIP1

NDFIP1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15602-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 15602-1-AP (same clone as 15602-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15602-1-AP (NDFIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15602-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 15602-1-AP (same clone as 15602-1-PBS)

  IP result of anti-NDFIP1 (IP:15602-1-AP, 3ug; Detection:15602-1-AP 1:300) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 15602-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 15602-1-AP (same clone as 15602-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using NDFIP1 antibody (15602-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15602-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15602-1-PBS cible NDFIP1 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDFIP1 Protéine recombinante Ag7985
• Nom complet: Nedd4 family interacting protein 1
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC004317
• Symbole du gène: NDFIP1
• Identification du gène (NCBI): 80762
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ndfip1 is an adaptor protein for the Nedd4 family of ubiquitin ligases and has been found to be upregulated in neurons after brain injury, including head trauma, stroke and metal toxicity. Recent studies confirmed the role of Ndfip1 as a regulator of iron metabolism and pointed out that Ndfip1 was involved in iron homeostasis by regulating the degradation of iron importer divalent metal transporter 1. Ndfip1 is a transmembrane protein that is localized to the Golgi and post-Golgi vesicles such as endosomes. It is an adaptor protein that recruits E3 ligases to ubiquitinate target proteins. It is ubiquitously expressed and contains 2 PY motifs, which interact with several Nedd4 family E3s to degradate proteins.

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