Anticorps Polyclonal de lapin anti-NDOR1/NR1

NDOR1/NR1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 11404-1-AP

Synonymes

NDOR1, NDOR1/NR1, Novel reductase 1, NR1

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Galerie de données de validation

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  WB analysis of human spleen using 11404-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11404-1-AP (NDOR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11404-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11404-1-AP (NDOR1/NR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 11404-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11404-1-AP (NDOR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 11404-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11404-1-AP (NDOR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11404-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11404-1-AP (NDOR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11404-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11404-1-AP (NDOR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 11404-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11404-1-AP (NDOR1/NR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 11404-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11404-1-AP (NDOR1/NR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11404-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NDOR1/NR1 antibody (11404-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 11404-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NDOR1/NR1 antibody (11404-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HaCaT, cellules HeLa, cellules Jurkat, cellules NIH/3T3, cellules U-251, tissu de thymus de souris, tissu hépatique humain, tissu pulmonaire humain, tissu splénique humain, tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 1 publications below

Informations sur le produit

11404-1-AP cible NDOR1/NR1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDOR1/NR1 Protéine recombinante Ag1909
• Nom complet: NADPH dependent diflavin oxidoreductase 1
• Masse moléculaire calculée: 606 aa, 68 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: BC015735
• Symbole du gène: NDOR1
• Identification du gène (NCBI): 27158
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	ACS Chem Neurosci; Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus.; Authors - Lei Wu
mouse	WB	Phytother Res; Rapid antidepressant-like effect of Fructus Aurantii depends on cAMP-response element binding protein/Brain-derived neurotrophic facto by mediating synaptic transmission.; Authors - Lei Wu
mouse	WB	Toxicology; PMID: 29800586; Authors - Xiaolong Wang
mouse	WB	Behav Brain Res; Down-regulation of NR2B receptors contributes to the analgesic and antianxiety effects of enriched environment mediated by endocannabinoid system in the inflammatory pain mice; Authors - Shukun Jiang
human	WB	J Mol Neurosci; PMID: 28730337; Authors - Xiaolong Wang
mouse	WB	Toxicol Res (Camb); Neurotoxicity, behavioral changes and gene-expression profile of mice exposed to SnS2 nanoflowers.; Authors - Disi Bai