Anticorps Monoclonal anti-NDP52

• Phare
• Validé par KD/KO

NDP52 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F2C2

N° de cat : 66401-1-PBS

Synonymes

CALCOCO2, Nuclear dot protein 52, Nuclear domain 10 protein 52, Calcium-binding and coiled-coil domain-containing protein 2, 2F2C2

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Galerie de données de validation

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  WB analysis using 66401-1-Ig (same clone as 66401-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66401-1-Ig (NDP52 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66401-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66401-1-Ig (same clone as 66401-1-PBS)

  WB result of NDP52 antibody (66401-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDP52 transfected HeLa cells. This data was developed using the same antibody clone with 66401-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66401-1-Ig (same clone as 66401-1-PBS)

  HeLa, Jurkat cells and human skeletal muscle tissue lysate were subjected to SDS PAGE followed by western blot with 66401-1-Ig (NDP52 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66401-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66401-1-Ig (same clone as 66401-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66401-1-Ig (NDP52 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66401-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66401-1-Ig (same clone as 66401-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66401-1-Ig (NDP52 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66401-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 66401-1-Ig (same clone as 66401-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using NDP52 antibody (66401-1-Ig, Clone: 2F2C2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66401-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66401-1-PBS cible NDP52 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NDP52 Protéine recombinante Ag2866
• Nom complet: calcium binding and coiled-coil domain 2
• Masse moléculaire calculée: 446 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC015893
• Symbole du gène: NDP52
• Identification du gène (NCBI): 10241
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa,

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