Anticorps Polyclonal de lapin anti-NDRG1

• Phare
• Validé par KD/KO

NDRG1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 26902-1-AP

Synonymes

CAP43, CMT4D, DRG 1, DRG1, GC4, HMSNL, N myc downstream regulated 1, NDR1, NDRG1, Protein NDRG1, PROXY1, RIT42, RTP, TARG1, TDD5

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Galerie de données de validation

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  WB analysis of HEK-293 using 26902-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26902-1-AP (NDRG1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 26902-1-AP

  WB result of NDRG1 antibody (26902-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDRG1 transfected HEK-293 cells.

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  WB analysis of HeLa using 26902-1-AP

  WB result of NDRG1 antibody (26902-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDRG1 transfected HeLa cells.

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  WB analysis of HeLa using 26902-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26902-1-AP (NDRG1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 26902-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26902-1-AP (NDRG1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26902-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26902-1-AP (NDRG1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 26902-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NDRG1 antibody (26902-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HeLa using 26902-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human NDRG1 (26902-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

26902-1-AP cible NDRG1 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDRG1 Protéine recombinante Ag25424
• Nom complet: N-myc downstream regulated 1
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC006260
• Symbole du gène: NDRG1
• Identification du gène (NCBI): 10397
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NDRG1 is a member of the N-myc down-regulated gene family which belongs to the alpha/beta hydrolase superfamily. NDRG1 is a highly conserved cytoplasmic protein involved in cell growth arrest and proliferation, cell differentiation, DNA damage response, heavy metal response, the hypoxia response, tumorigenesis, and metastasis. NDRG1 is a potent metastasis suppressor in multiple tumor-types. NDRG1 appears to serve as a putative tumor suppressor and is down-regulated in colorectal, gastric, cervical, ovarian and prostate cancers.

Publications

Species	Application	Title
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
human	WB	Nucleic Acids Res; TARG1 protects against toxic DNA ADP-ribosylation.; Authors - Callum Tromans-Coia ; KD Validated
human	WB,IHC	Cell Oncol (Dordr); LINC01419 promotes cell proliferation and metastasis in hepatocellular carcinoma by enhancing NDRG1 promoter activity.; Authors - Hao Dang
human	WB	Gynecol Oncol; Deferasirox shows inhibition activity against cervical cancer in vitro and in vivo.; Authors - Nan Zhou
human	WB,IHC	PeerJ; LINC00844 promotes proliferation and migration of hepatocellular carcinoma by regulating NDRG1 expression.; Authors - Wei Zhou
human	WB	J Oncol; Ferritinophagic Flux Was a Driving Force in Determination of Status of EMT, Ferroptosis, and NDRG1 Activation in Action of Mechanism of 2-Pyridylhydrazone Dithiocarbamate S-Acetic Acid.; Authors - Hao Li ; KD Validated