Anticorps Polyclonal de lapin anti-NDRG2

• Phare
• Validé par KD/KO

NDRG2 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 12015-1-AP

Synonymes

NDRG family member 2, N-myc downstream-regulated gene 2 protein, Protein NDRG2, Protein Syld709613, SYLD

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Galerie de données de validation

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  WB analysis of mouse brain using 12015-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12015-1-AP (NDRG2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12015-1-AP

  WB result of NDRG2 antibody (12015-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-NDRG2 transfected HepG2 cells.

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  IP experiment of rat brain using 12015-1-AP

  IP result of anti-NDRG2 (IP:12015-1-AP, 4ug; Detection:12015-1-AP 1:1000) with rat brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 12015-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12015-1-AP (NDRG2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12015-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12015-1-AP (NDRG2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HepG2, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

12015-1-AP cible NDRG2 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDRG2 Protéine recombinante Ag2629
• Nom complet: NDRG family member 2
• Masse moléculaire calculée: 371 aa, 39 kDa
• Poids moléculaire observé: 41-43 kDa
• Numéro d’acquisition GenBank: BC010458
• Symbole du gène: NDRG2
• Identification du gène (NCBI): 57447
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NDRG2, or N-myc downstream-regulated gene 2, is a tumor suppressor gene that plays a significant role in cancer development and progression. It is associated with tumor incidence, progression, and metastasis, and is known to regulate tumor-associated genes. NDRG2 is part of the NDRG family, which includes NDRG1, NDRG2, NDRG3, and NDRG4. These proteins are characterized by an esterase/lipase/thioesterase active site serine and an α/β hydrolase fold of approximately 220 amino acids, although they do not exhibit enzyme activity.

Publications

Species	Application	Title
mouse,human	IHC	Front Immunol; Single-cell protein activity analysis reveals a novel subpopulation of chondrocytes and the corresponding key master regulator proteins associated with anti-senescence and OA progression; Authors - Zhao Guang
human	WB	J Ovarian Res; NDRG2 gene expression pattern in ovarian cancer and its specific roles in inhibiting cancer cell proliferation and suppressing cancer cell apoptosis.; Authors - Fenhong Kang

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