Anticorps Polyclonal de lapin anti-NDST1

• Phare
• Validé par KD/KO

NDST1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 26203-1-AP

Synonymes

HSST, HSST1, N HSST 1, NDST 1, NDST1, NST1

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Galerie de données de validation

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  WB analysis using 26203-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26203-1-AP (NDST1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 26203-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26203-1-AP (NDST1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 26203-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26203-1-AP (NDST1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 26203-1-AP

  IP result of anti-NDST1 (IP:26203-1-AP, 4ug; Detection:26203-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, tissu cardiaque de souris, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

26203-1-AP cible NDST1 dans les applications de WB, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDST1 Protéine recombinante Ag24422
• Nom complet: N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1
• Masse moléculaire calculée: 882 aa, 101 kDa
• Poids moléculaire observé: 68-70 kDa
• Numéro d’acquisition GenBank: BC012888
• Symbole du gène: NDST1
• Identification du gène (NCBI): 3340
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human,rat	WB,FC	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB	J Extracell Vesicles; Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease; Authors - Rong Yang
human	WB	Adv Sci (Weinh); Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia; Authors - Tiemei Li
mouse	WB	Exp Mol Med; PGC-1α mediates migrasome secretion accelerating macrophage-myofibroblast transition and contributing to sepsis-associated pulmonary fibrosis; Authors - Yawen Peng
human	WB,IF	Ann Neurol; Parkinson's Disease Derived Exosomes Aggravate Neuropathology in SNCA*A53T Mice.; Authors - Yang Huang ; KD Validated
human	WB	PLoS Pathog; N-Glycans and sulfated glycosaminoglycans contribute to the action of diverse Tc toxins on mammalian cells.; Authors - Nan Song ; KO Validated

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