Anticorps Recombinant de lapin anti-NDUFA1

NDUFA1 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251084A5

N° de cat : 86470-1-PBS

Synonymes

CI MWFE, CI-MWFE, Complex I MWFE, Complex I-MWFE, MWFE

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Galerie de données de validation

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  WB analysis using 86470-1-RR (same clone as 86470-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86470-1-RR (NDUFA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86470-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 86470-1-RR (same clone as 86470-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86470-1-RR (NDUFA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86470-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 86470-1-RR (same clone as 86470-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86470-1-RR (NDUFA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86470-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 86470-1-RR (same clone as 86470-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 86470-1-RR (NDUFA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86470-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 86470-1-RR (same clone as 86470-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 86470-1-RR (NDUFA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86470-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86470-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86470-1-RR against Human NDUFA1 were performed. The affinity constant is 0.588 nM.

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Informations sur le produit

86470-1-PBS cible NDUFA1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NDUFA1 Protéine recombinante Ag7206
• Nom complet: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5kDa
• Masse moléculaire calculée: 7.5 kDa
• Poids moléculaire observé: 8 kDa
• Numéro d’acquisition GenBank: BC000266
• Symbole du gène: NDUFA1
• Identification du gène (NCBI): 4694
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NDUFA1, also known as NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1, it's part of the NADH dehydrogenase (ubiquinone) 1 alpha subcomplex. The NDUFA1 gene encoding the MWFE polypeptide is located on the X chromosome. The NDUFA1 gene product (MWFE protein) is essential for activity of complex I in mammalian mitochondria, and it is predicted to be a membrane protein (PMID: 10200266).

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