Anticorps Polyclonal de lapin anti-NDUFA10

NDUFA10 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 32947-1-PBS

Synonymes

CI-42kD, Complex I-42kD, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial, NADH:Ubiquinone Oxidoreductase Subunit A10, NADH-ubiquinone oxidoreductase 42 kDa subunit

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Galerie de données de validation

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  WB analysis using 32947-1-AP (same clone as 32947-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 32947-1-AP (NDUFA10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32947-1-PBS in a different storage buffer formulation.

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  WB analysis using 32947-1-AP (same clone as 32947-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 32947-1-AP (NDUFA10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32947-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 32947-1-AP (same clone as 32947-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 32947-1-AP (NDUFA10 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32947-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 32947-1-AP (same clone as 32947-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using NDUFA10 antibody (32947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 32947-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 32947-1-AP (same clone as 32947-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFA10 antibody (32947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 32947-1-PBS in a different storage buffer formulation.

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Informations sur le produit

32947-1-PBS cible NDUFA10 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDUFA10 Protéine recombinante Ag37576
• Nom complet: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC003417
• Symbole du gène: NDUFA10
• Identification du gène (NCBI): 4705
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NDUFA10 (NADH:ubiquinone oxidoreductase subunit A10) is a subunit of mitochondrial complex I of the electron transport chain, encoding a protein with NADH dehydrogenase and oxidoreductase activity. This protein transfers electrons from NADH to the respiratory chain. Mutations in the NDUFA10 gene are associated with Leigh syndrome, a common mitochondrial energy metabolism disorder in children. Additionally, the expression levels of NDUFA10 are related to the occurrence and prognosis of various diseases. For example, in Alzheimer's disease, its low expression is associated with increased dementia severity. In prostate cancer, low expression of NDUFA10 predicts a poor prognosis.

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