Anticorps Monoclonal anti-NDUFA4L2

NDUFA4L2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

1G1H10

Avis (2)

N° de cat : 66050-1-Ig

Synonymes

NUOMS, NADH-ubiquinone oxidoreductase MLRQ subunit homolog, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2, 1G1H10

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Galerie de données de validation

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  WB analysis of HeLa using 66050-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66050-1-Ig (NDUFA4L2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66050-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66050-1-Ig (NDUFA4L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66050-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66050-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 66050-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 66050-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66050-1-Ig (NDUFA4L2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of OS-RC-2 using 66050-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed OS-RC-2 cells using NDUFA4L2 antibody (66050-1-Ig, Clone: 1G1H10 ) at dilution of 1:1200 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  IF Staining of HUVEC using 66050-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using NDUFA4L2 antibody (66050-1-Ig, Clone: 1G1H10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 66050-1-Ig

  Immunofluorescent analysis of Hela cells, using NDUFA4L2 antibody 66050-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

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  IF Staining of MCF-7 using 66050-1-Ig

  Immunofluorescent analysis of MCF-7 cells, using NDUFA4L2 antibody 66050-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de carcinome à cellules rénales humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HUVEC, cellules HeLa, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

66050-1-Ig cible NDUFA4L2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NDUFA4L2 Protéine recombinante Ag9233
• Nom complet: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2
• Masse moléculaire calculée: 87 aa, 10 kDa
• Poids moléculaire observé: 10 kDa
• Numéro d’acquisition GenBank: BC011910
• Symbole du gène: NDUFA4L2
• Identification du gène (NCBI): 56901
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NDUFA4L2, also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.

Publications

Species	Application	Title
human	IHC	Elife; Molecular classification and tumor microenvironment characteristics in pheochromocytomas; Authors - Sen Qin
human	WB	Cancer Biol Ther; NDUFA4L2 reduces mitochondrial respiration resulting in defective lysosomal trafficking in clear cell renal cell carcinoma; Authors - Jaclyn M Kubala

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kristian (Verified Customer) (06-07-2021)

The cells were stained with anti-NDUFA4l2 (green), Phalloidin (purple), and DAPI (blue). The NDUFA4L2 is localized in the mitochondria of the RCC4 cells.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: RCC4

NDUFA4L2 Antibody Immunofluorescence validation (1:200 dilution) in RCC4 (Cat no:66050-1-Ig)

FH

Daniel (Verified Customer) (04-16-2019)

Specific band in hypoxic mouse kidney. Additional band for Ndufa4.Note: The upper part of the blot (>25 kDa) was stained for a hypoxic marker (Car9).

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse kidney, hypoxic (lane1), normoxic (lane2)

NDUFA4L2 Antibody Western Blot, validation (1:1000 dilution) in Mouse kidney, hypoxic (lane1), normoxic (lane2) (Cat no:66050-1-Ig)

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