Anticorps Recombinant de lapin anti-NDUFA4L2

NDUFA4L2 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241622B12

N° de cat : 84498-1-PBS

Synonymes

NUOMS, NADH-ubiquinone oxidoreductase MLRQ subunit homolog, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2, 241622B12

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Galerie de données de validation

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  WB analysis using 84498-1-RR (same clone as 84498-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84498-1-RR (NDUFA4L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84498-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 84498-1-RR (same clone as 84498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84498-1-RR (NDUFA4L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84498-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 84498-1-RR (same clone as 84498-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84498-1-RR (NDUFA4L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84498-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84498-1-RR (same clone as 84498-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFA4L2 antibody (84498-1-RR, Clone: 241622B12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84498-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84498-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84498-1-RR against Human NDUFA4L2 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84498-1-PBS cible NDUFA4L2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NDUFA4L2 Protéine recombinante Ag9600
• Nom complet: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4-like 2
• Masse moléculaire calculée: 87 aa, 10 kDa
• Poids moléculaire observé: 10 kDa
• Numéro d’acquisition GenBank: BC011910
• Symbole du gène: NDUFA4L2
• Identification du gène (NCBI): 56901
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NDUFA4L2 (NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4-like 2), also named as NUOMS, is a HIF-1α target gene that localizes to the mitochondria and belongs to the complex I NDUFA4 subunit family. It downregulates oxygen consumption and Complex I activity in hypoxia. NDUFA4L2 is involved in hypoxic adaptation by decreasing mitochondrial ROS production.

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