Anticorps Recombinant de lapin anti-NDUFA5

NDUFA5 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242860B10

N° de cat : 85187-1-PBS

Synonymes

242860B10, B13, CI 13KD B, CI-13kD-B, Complex I 13kD B

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Galerie de données de validation

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  WB analysis using 85187-1-RR (same clone as 85187-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85187-1-RR (NDUFA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85187-1-PBS in a different storage buffer formulation.

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  WB analysis using 85187-1-RR (same clone as 85187-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85187-1-RR (NDUFA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85187-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85187-1-RR (same clone as 85187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85187-1-RR (NDUFA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85187-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85187-1-RR (same clone as 85187-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85187-1-RR (NDUFA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85187-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01899-1

  Cytometric bead array standard curve of MP01899-1, NDUFA5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85187-1-PBS. Detection antibody: 85187-4-PBS. Standard: Ag9995. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 85187-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85187-1-RR against Human NDUFA5 were performed. The affinity constant is 0.378 nM.

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Informations sur le produit

85187-1-PBS cible NDUFA5 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: NDUFA5 Protéine recombinante Ag9995
• Nom complet: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa
• Masse moléculaire calculée: 13 kDa
• Poids moléculaire observé: 13 kDa
• Numéro d’acquisition GenBank: BC000813
• Symbole du gène: NDUFA5
• Identification du gène (NCBI): 4698
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NDUFA5, also named as CI-13kD-B and NDUA5, belongs to the complex I NDUFA5 subunit family. It is accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which functions in the transfer of electrons from NADH to the respiratory chain. NDUFA5,an enrichment of mitochondrial protein, can be used as a mitochondrial marker protein(PMID:21360670).

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