Anticorps Polyclonal de lapin anti-NDUFAF3

NDUFAF3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25621-1-PBS

Synonymes

NDUF3, E3 3, C3orf60, 2P1

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Galerie de données de validation

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  WB analysis using 25621-1-AP (same clone as 25621-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25621-1-AP (NDUFAF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25621-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 25621-1-AP (same clone as 25621-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25621-1-AP (NDUFAF3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25621-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 25621-1-AP (same clone as 25621-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NDUFAF3 antibody (25621-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25621-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25621-1-PBS cible NDUFAF3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDUFAF3 Protéine recombinante Ag22514
• Nom complet: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 3
• Masse moléculaire calculée: 184 aa, 20 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC002873
• Symbole du gène: NDUFAF3
• Identification du gène (NCBI): 25915
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NDUFAF3 has been assigned to be an assembly factor of the Q-module because it physically associates with Q-module subassemblies during complex I biogenesis (PMID: 27986404).

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